对乙型肝炎表面抗原弱阳性结果进行确认试验的应用研究

Research on the clinical application of the confirmatory test for samples with weak positive HBsAg

目的评估抗体中和确认试验在乙型肝炎表面抗原（HBsAg）低值弱阳性标本中的应用。方法将由微粒子酶免疫发光法（MEIA）检测的HBsAg结果（S/CO）在1.0-10.0之间的111份弱阳性血清标本进行抗体中和确认试验,并进行乙型肝炎病毒（HBV）酶联免疫吸附试验（ELISA）,对比各试验结果间的差异。结果抗体中和确认试验阳性92例（82.9%）,ELISA检测阳性47例（42.3%）,2项试验检测结果间差异有统计学意义（P=0.033）。结论对HBsAg低值弱阳性标本,ELISA检测漏检率很高,敏感性和特异性均不佳;抗体中和确认试验可做为HBsAg弱阳性标本进一步确认的手段。

Objective To study the clinical application of the confirmatory test for samples with weak positive hepatitis B surface antigen（HBsAg）.Methods 111 weak positive HBsAg serum samples,which were screened by microparticle enzyme immunoassay（MEIA） and their S/CO were 1.0-10.0,were retested by antibody neutralization confirmatory test and Hepatitis B virus（HBV） enzyme-linked immunosorbent assay（ELISA）.The differences between the results of these tests were compared.Results The positive results of antibody neutralization confirmatory test and ELISA were 92（82.9%） and 47（42.3%） respectively.The difference between the results of the two tests had statistical significance（P=0.033）.Conclusions For the samples with weak positive HBsAg,ELISA has high omission rate,poor sensitivity and specificity.Antibody neutralization confirmatory test can be used as the further means to confirm the samples with weak positive HBsAg.