microRNA在大鼠急性心肌梗死模型的差异表达及其功能分析

Identification of differentially expressed microRNAs in response to AMI in rat

目的应用microarray筛选在急性心肌梗死缺血心肌中差异表达的microRNA(miRNA),用生物信息学方法分析miRNA的转录因子和靶基因功能,探索其相关性,为进一步阐明miRNA在急性心肌梗死过程中的病理生理作用打下基础。方法20只雄性SD大鼠,通过前降支动脉结扎法建立急性心肌梗死模型,模型成功后随机分为心肌梗死后2d组,7d组,14d组,每组5只动物。另设对照组(n=5)。提取缺血心肌的总RNA进行芯片检测,应用实时定量RT-PCR法验证芯片结果的可靠性。结果microarray检测获得17个与急性心肌梗死相关的miRNA,其中9个miRNA表达显著上调,8个miRNA表达显著下调,miRNA靶基因和转录因子的功能涉及心肌再生\血管新生\心肌肥大等等。结论microarray筛选得到的与急性心肌梗死相关的差异表达的miRNA可能作为心肌梗死新的生物标记物和治疗靶点。

Objective To screen and identify the miRNA differential expression profile in response to acute myocardial infarction in rat by the miRNA array technique to make further study on the function of miRNA in pathogenesis of AMI.Methods Total RNA was extracted from ischemial myocardium and normal myocardium.Small miRNA was isolated from total RNA,labeled with Cy3 and hybridized on miRNA array.Real time quantitative PCR was applied to verify the reliability of miRNA array results.Results By significance analysis of microarray（SAM）based on microarray screening,17 AMI related miRNAs were obtained.9 up-regulated and 8 down-regulated miRNAs were observed.The most significant up-regulated miRNAs were miR-31 and miR-214,while miR-499 and miR-126 were significantly down-regulated.Conclusion miRNA differential expression profile of AMI was obtained,which may be related to pathogenesis and development of AMI.