小牛血去蛋白提取物对小鼠能量代谢的影响

Effect of deproteinized extract of calf blood on energy metabolism in mice

目的观察小牛血去蛋白提取物在改善小鼠大脑和心肌能量代谢的作用。方法应用葡萄糖氧化酶法测定小鼠血糖、心肌和大脑皮层葡萄糖含量。制备小鼠急性缺氧性脑缺血模型,应用紫外分光光度法测定小鼠血液和大脑皮层中的乳酸含量。结果小牛血去蛋白提取物可降低血糖浓度,同时升高心肌内葡萄糖含量。小鼠急性缺氧性脑缺血后,血液和皮层中乳酸浓度显著升高,小牛血去蛋白提取物可降低乳酸浓度。结论小牛血去蛋白提取物能够改善小鼠大脑和心肌能量代谢,对于治疗缺氧性缺血疾病有一定的意义。

Objective To observe the effect of deproteinized extract of calf blood on brain and my- ocardial energy metabolism in mice. Methods Glucose oxidase method was used to assay the concentrations of glucose in blood, myocardium and cerebral cortex. Uhraviolet spectrophotometric method was used to de- tect the eoncertration of lactic acid in blood and cerebral cortex after the mice models of acute cerebral ische- mia-hypoxia were produced. Results Compared with control group, blood glucose was decreased and glu- cose in myocardium increased significantly after administration of deproteinized extract of calf blood. The concentrations of lactic acid in blood and cerebral cortex of mice were increased significantly under the acute cerebral ischemia-hypoxia and were reversed after administration of deproteinized extract of calf blood. Con- clusion Deproteinized extract of calf blood could be used to treat cerebral ischemia-hypoxia with the effect of improving energy metabolism of brain and myocardium.