“中花11”水稻谷蛋白Gt1基因克隆及蜡质基因启动子引导Gt1基因表达载体的构建

Cloning of Gt1 gene in "Zhonghua 11" Rice and construction of expression vector with Gt1 gene under the Waxy promoter

谷蛋白含量及分布对水稻营养品质起着至关重要的作用.利用PCR技术从"中花11"水稻基因组成功克隆到谷蛋白Gt1基因,再分别以PCR技术和限制酶酶切从p13W4载体上获得水稻蜡质基因(Waxy)启动子、第一内含子和NOS终止子,并以pCAMBIA1301双元载体为骨架,成功构建了由Waxy启动子引导的水稻Gt1基因表达载体,为今后利用转基因技术改良水稻稻米营养品质奠定了基础.

Quantity and distribution of glutelin play an important role in the quality of rice nutrition. The glutelin Gtl gene was cloned from the genomie DNA of ＂Zhonghua 11＂ rice, and then the promoter and first intron of rice Waxy gene and NOS terminator were gotten from p13W4 vector respectively by using the PCR and restriction enzyme digestion. The expression vector with Gtl gene under the Waxy promoter was constructed successfully on the pCAMBIA1301 binary vector as a basic frame. The experiment will lay a foundation for studying on improving nutritional quality of rice through transgenic technology.