摘要
运用Box-Behnken中心组合响应面分析法优化胰蛋白酶法提取核桃多肽的工艺条件,探讨了酶解过程中酶解pH值、温度和加酶量对核桃多肽提取的影响。优化工艺方案为:酶解pH7.0,温度45℃,加酶量5000U·g-1,其氨基氮的平均含量达到0.206%。
Box-Behnken center-united experiment design attached to response surface method was applied to optimize the extraetion condition for walnut peptide by enzymatic way. The parameters such as pH value, reaetion temperature and enzymatic quantity were discussed.The results indicated that the optimal parameters of extraetion technology for the content of walnut peptide by cellulase were pH 7.0, reaction temperature 45℃ and enzymatic quantity 5000 U.g^-1.The maximal content of Amino nitrogen in average was 0.2061% in this paper.
出处
《中国食物与营养》
2010年第4期52-55,共4页
Food and Nutrition in China
关键词
核桃多肽
胰蛋白酶
响应面法
Walnut Peptide
Cellulase
Response Surface Method （RSM）
