慢性复合应激对大鼠血清脂联素及肝脏脂联素受体-2表达的影响

Effects of chronic compound stress on serum adiponectin level and adipoR2 expression in rats.

目的研究慢性复合应激对大鼠血清脂联素水平及肝脏脂联素受体-2（AdipoR2）表达的影响。方法将40只SD大鼠随机分为应激组和对照组，每组20只。应激组实施8周的慢性复合应激刺激，对照组在此期间正常饲养。在实验的第8周将大鼠断头处死，取血检测脂联素、血糖、胰岛素抵抗（IR）、糖化血红蛋白（HbA1C）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL—C）、甘油三酯、总胆固醇含量。结果①8周后，应激组与对照组旷场实验得分和24h尿皮质醇差异有统计学意义[（32．0±8．6）、（52．0±12．7）分，（5．8±0．4）、（5．3±0．1）ng／L，P〈0．05或〈0．01]。②8周后应激组与对照组脂联素、总胆固醇，甘油三酯、HDL—C、1nIR、HbA，C差异有统计学意义[（4．97±0．57）、（5．63±0．49）mg／L，（1．44±0．24）、（1．07±0．23）mmo]／L，（0．47±0．09）、（0．39±0．05）mmol／L，（0．76±0．17）、（0．89±0．15）mmol／L，（1．89±0．46）、（1．41±0．32），（7．84±1．72）％、（6．08±1．56）％，t值分另q为-3．927、4．978、3．475、-2．564、3．831、3．390，P〈0．05或〈0．01]。③8周后应激组和对照组大鼠肝脏AdipoR2 mRNA相对表达分别为0．67±0．04、1．00±0．11，蛋白相对表达分别为0．73±0．02、1．00±0．04，差异有统计学意义（t值分别为-12．609、-27．000，P均〈0．01）。脂联素与HbA1C、LDL-C、IR显著负相关（r值分别为-0．26、-0．88、-0．37，P〈0．05或P〈0．01），与HDL—C显著正相关（r=0．78，P〈0．01）。结论慢性复合应激使大鼠血清脂联素水平和肝脏AdipoR2表达降低。

Objective To explore the effect of chronic compound stress on serum adiponectin level and AdipoR2 expression in rats. Methods Forty male Sprague-Dawley rats were randomly divided into two groups：the stress group underwent chronic compound stress and the control group was fed normally. After 8 weeks of stress or feeding, the serum adiponectin, blood glucose,insulin resistance and HbA, C, HDL-C, LDL-C, triglyceride and total cholesterol were measured. Results ①After 8 weeks of stress, the score of open-field behaviors and 24-h urocortisol were significantly different between the stress and control groups（32. 0 ±8.6 vs. 52.0 ± 12. 7,5.8 ±0. 4 vs. （5.3 ± 0. 1 ） ng/L, repectively ; P 〈 0. 05 or 0. 01 ） ;②After 8 weeks of stress, the adiponectin, total cholesterol, triglyceride, HDL-C, lnlR and HbA1C were significantly different between the two groups （ P 〈 0. 05 or 0. 01 ） ; ③After 8 weeks of stress, there were significant difference on the expression of AdipoR2 mRNA and protein between the two groups （P 〈 0. 01 ）, with the expression of AdipoR2 mRNA of 0. 67 ±0. 04 and 1.00 ± 0. 11 and the expression of protein of 0. 73 ± 0. 02 and 1.00 ± 0. 04 in the stress and control group, respectively. Adiponectin was negatively related with HbA1C, LDL-C and IR （r = -0. 26, -0. 88 and -0. 37,P 〈 0.05 or 0. 01 ）, and positively related with HDL-C （r = 0. 78 ,P 〈 0. 01 ）. Conclusions In rats, chronic compound stress decreases the level of serum adiponectin and the expression of AdipoR2 in liver.