摘要
以紫薇嫩茎产生的愈伤组织为外植体,通过正交试验设计,研究不同培养基对紫薇丛生芽诱导、增殖和生根的影响。结果表明:不同浓度的NAA6、-BA及KT激素组合对紫薇愈伤组织丛生芽诱导培养的影响显著,最佳诱导培养基为:MS+NAA 0.05 mg/L+6-BA 0.5 mg/L+KT 0.5 mg/L;不同浓度激素组合对紫薇丛生芽增殖培养影响极显著,最佳增殖培养基为:MS+NAA 0.05 mg/L+6-BA 2.0 mg/L+KT 1.0 mg/L;不同培养基对紫薇生根培养的影响较显著,生根培养适合的培养基为:1/2MS+NAA 0.5 mg/L+6-BA 0.1 mg/L。
By orthogonal experiment the tendergreen stems were used as explants to study the effect of different medium on cluster shoots induction, proliferation and rooting experiment of Lagerstroemia indica. The results indicated that the significant impact to Lagerstroemia indica cluster shoots induction cultivated on different concentrations of hormone combinations. The optimal induction medium was MS＋NAA 0. 05 mg/L＋6-BA 0. 5 mg/L＋KT 0. 5 mg/L. There was a very significant impact to Lagerstroemia indica cluster shoots proliferation cultivated on different concentrations of hormone combinations. The optimal proliferation medium was MS＋NAA 0. 05 mg/L＋6-BA 2. 0 rng/L＋KT 1.0 mg/L. There was great impact to Lagerstroemia indica rooting cultivated in different treatments. The adaptive rooting medium was 1/2MS＋NA.A 0. 5 mg/L＋6-BA 0. 1 mg/L.
出处
《北方园艺》
CAS
北大核心
2010年第8期149-151,共3页
Northern Horticulture
基金
湖南省科技厅科技计划资助项目(2008NK3116)
关键词
紫薇
丛生芽诱导
丛生芽增殖
生根培养
Lagerstroemia indica
cluster shoots induction
cluster shoots proliferation
rooting cultivation
