影响千层金腋芽萌发及愈伤组织诱导因子初探

Preliminary Studying Factor on Effects Germination and Calls Inducing of Melaleuca bracteata

为建立千层金高效的离体再生体系,用新生顶芽和侧芽为外植体,研究了灭菌剂、取材季节、植物生长调节剂种类和浓度、硝酸银浓度对千层金腋芽萌发及愈伤组织诱导的影响。结果表明:与使用0.1%氯化汞64%的存活率相比,5%的次氯酸钙是最环保有效的灭菌剂,用其浸泡5 min,有60%存活率;在春秋两季取材,平均诱导率在92.0%以上;最适宜的诱导培养基是MS+KT 1.0 mg/L+NAA 0.1 mg/L;使用3%的硝酸银,与对照相比,诱导率从70%提高到92%,玻璃化率从33.7%下降到10%。

The regeneration of Melaleuca bracteata the effects of the disinfectant, drawing explants period, the types and concentration of plant growth regulators and silver nitrate on its induction germination and calls were studied with the buds and tender stem segments as explants in this paper. The results showed that 0. 1% mercuric chloride with the use of the survival rate of 64% compared to 5 % of the calcium hypochlorite is the most environmentally friendly and effective disinfectant, it has the survival rate of 60%when socked 5 mira. The best tissue culture period is the spring and autumn, with an average induction rate of 92.0% over. The best suitable inducting medium was MS＋KT 1.0 mg/L＋NAA 0. 1 mg/L. The use of 3% silver nitrate, compared with the control, the induction rate from 70% to 92%, vitrification rate from 33.7% down to 10%.