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阔褶水蛙皮肤组织抗菌肽latouchiin-1和latouchiin-2的基因克隆和序列分析 被引量：1

Molecule Cloning and Sequence Analysis of the cDNA Encoding Antimicrobial Peptides latouchiin-1 and latouchiin-2 from Hylarana latouchii Skin Tissue
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摘要 为了从生物体中寻找新颖的抗菌肽类药物分子,以阔褶水蛙(Hylarana latouchii)为研究材料,运用3′RACE技术成功从其皮肤组织中筛选出2个抗菌肽前体cDNA编码序列,经blast生物信息学分析,确定其为首次发现,推导出的这2条新颖的抗菌肽序列,命名为latouchiin-1和latouchiin-2。 In this study,we used Hylarana latouchii as the material to find novel antimicrobial peptides from n- ative organism. Two different cDNAs encoding two novel antimicrobial peptide precursors were cloned by 3＇ RACE technique from skin of H. latouchii. There was no homologous sequence with these two cDNA sequences by blasting analyse. These two novel antimicrobial peptide precursors were named as latouchiin-1 and latouchiin-2.
作者 王辉 郧海丽 赵军锋 王多 冉然 胡玉红
机构地区 河北师范大学实验中心 河北师范大学生命科学学院 石家庄学院化工学院 河北师范大学汇华学院
出处 《华北农学报》 CSCD 北大核心 2010年第2期12-17,共6页 Acta Agriculturae Boreali-Sinica
基金 河北师范大学青年基金(L2007Q32)
关键词 阔褶水蛙 抗菌肽 分子克隆 皮肤组织 Hylarana latouchii Antimicrobial peptides Molecule cloning Skin tissue
分类号 Q785 [生物学—分子生物学]
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参考文献17

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二级参考文献63

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华北农学报
2010年 第2期

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