传染性法氏囊病病毒VP2蛋白B细胞抗原表位免疫小鼠试验

Mice Immunization with the B-cell Epitopes on the VP2 Protein of Infectious Bursal Disease Virus

为检测Ⅰ型IBDVVP2蛋白线性B细胞抗原表位肽PJ14和PJ5的免疫原性,将人工合成并纯化的PJ14和PJ5分别与牛血清白蛋白(BSA)和卵清白蛋白(OVA)偶联;将与BSA偶联的PJ14和PJ5分别免疫BALB/c小鼠,然后以与OVA偶联的PJ14和PJ5作为间接酶联免疫吸附试验抗原,分别测定免疫鼠血清中抗相应多肽的抗体。结果显示,免疫小鼠产生了抗PJ14和PJ5的抗体,抗体持续期达21周。表明PJ14和PJ5具有免疫原性。

The peptides of the linear B-cell epitopes PJ14 and PJ5 on the VP2 protein of infectious bursal disease virus（IBDV） were synthesized and purified, then were conjugated to carrier protein BSA or OVA respectively. The BSA-conjugated PJ14 and PJ5 were inoculated into the BALB/c mice respectively. The OVA-conjugated PJ14 was used as the antigen to detect the anti-PJ14 antibody in the serum of the immunized mice,and so was the OVA-conju- gated PJS. The results showed that the PJ14 and PJ5 after the conjugation to BSA stimulated the production of the an- ti-PJ14 and anti-PJ5 antibody respectively, and that the antibodies lasted for twenty-one weeks. It indicated the immu- nogenicity of the PJ14 and PJS.