花粉萌发特性对扁桃不亲和强度差异的影响

Effect on Self-fertilize Un-appetency Intensity Difference in Almond About Pollen Bourgeon Characteristic

以新疆主栽扁桃8个品种和10个杂交组合为对象,研究其自交以及杂交组合的花柱离体培养花粉管的生长情况,花柱蛋白浓度对不同品种花粉的萌发生长抑制作用的影响,并分析其对扁桃自交不亲和性影响的强度差异。结果表明:在花柱1/3处的花粉管数量差别较小,而在花柱1/2处花粉管数量显著不同。花柱蛋白在低浓度下对花粉萌发和伸长没有抑制作用,蛋白浓度超过2.0μg/μL时才发生轻微的抑制作用,并且随蛋白浓度的升高抑制作用增强,表明高浓度的花柱蛋白比低浓度的花柱蛋白更能诱导不亲和的发生。

Study on 8 breed and 10 cross-fertilize composes in almond. The growth situation of pollen tube by style isolated culture of self-fertilize and cross-fertilize composes. The results showed that the difference of pollen tube number in 1/3 of style between appetency and un-appetency varieties composes was insignificant, but the difference in 1/2 of style was evident. However the number of pollen tube number in 1/2 of style could not distinguish appetency and un-appetency composes strictly. So number of pollen tube growing from the base of style can be the index to appraise the compatibility among almond varieties in style isolated culture. The inhibition extent that stylus protein to the grown of pollen tube of different varieties was obviously different. However, the exhibition of inhibition stylus protein to almond was identical： there was no inhibition stylus protein in low density which was 1.0μg/μL to the germination and elongation of almond pollen. The slight inhibition happens when the density of stylus protein was more than 2. 0μg/μL and strengthens with density of stylus protein rising. It indicated that high density stylus protein can induce the emergence compatibility easier than low density stylus protein.