摘要
为了进一步研究富勒烯家族及其衍生物引起细胞自噬的现象,运用荧光显微镜、透射电子显微镜、蛋白免疫印迹等技术,对水溶性的纳米C70,C60Cl6和C60Ph6诱导的细胞自噬现象进行了初步探讨.实验结果显示,C70,C60Cl6和C60Ph6稳定分散于水中形成的纳米颗粒,均能诱导HeLa细胞产生自噬现象,揭示了富勒烯家族及其衍生物在生物医药领域更广泛的研究和应用价值.
In order to further study the autophagy induced by fullerene family and its derivatives,nano-C70,C60Cl6 and C60Ph6 were selectively investigated by means of fluorescence microscope,transmission electron microscope and western blot.The results show that,nano-C70,C60Cl6 and C60Ph6 can form crystal structures in water and induce HeLa cells into autophagy process,which indicates that fullerene family and its derivatives might play a new role in the biomedical field.
基金
国家重点基础研究发展(973)计划(2006CB933300
2009CB930300)资助
