反硝化除磷脱氮系统中DPB的驯化富集培养

Cultivation and Enrichment of Denitrifying Phosphorus Removal Bacteria (DPB) in Denitrifying Biological Nutrient Removal Process

反硝化同时脱氮除磷系统中反硝化除磷菌(DPB)的培养驯化状况,将直接影响污水中氮磷等营养元素的同时去除效率以及系统的高效稳定运行,为此本实验研究设计了一套以实际生活污水为处理对象的双污泥反硝化脱氮除磷工艺流程,采用逐渐过渡的培养方式,为DPB创造良好的厌氧/缺氧交替环境,即创造特定的适合DPB生存的环境条件让其进行自然选择,以筛选出来需要的DPB菌.结果表明,通过15d的间歇曝气的厌氧/好氧(A/O)运行方式可以对PAOs进行快速诱导;第二阶段,通过好氧曝气时间的逐渐减少,缺氧段投加硝酸氮的厌氧/好氧/缺氧(A/O/A)运行模式,25d左右可达到强化诱导反应器里面的DPB占PAOs的比例;最后让DPB在严格的厌氧/缺氧交替环境下进行富集培养19d,通过这种逐渐过渡培养的方式获得了对所需要的DPB菌的成功诱导富集,该菌的成功驯化培养为市政生活污水中的氮磷同时高效稳定去除提供了一种新方法.

The cultivation of the denitrifying phosphorus removal bacteria （DPB） in biological nutrient removal system will directly affect the removal efficiency of the nutrients in wastewater such as nitrogen and phosphorus,and the stability of the process. For this reason,a new BNR process,which is called double-sludge denitrifying-nitrogen and phosphorus removal process,was designed. In order to create a good anaerobic/anoxic alternating environment for DPB,gradual transition of training methods were used to make DPB natural selection,and then finally,screened the dominated DPB bacteria. The results indicated that the anaerobic/aerobic （A/O） operation mode can induce induction of PAOs rapidly by 15 days intermittent aeration. The proportion of DPB/PAOs in the reactor was aggrandized in the second phase（about 25 days＇ inducement） by reducing the aeration time in aerobic phase gradually and adding nitrate in anoxic phase,which is called anaerobic/aerobic/anoxic （ A/O/A） mode; finally,the strictly alternating environmental anaerobic/anoxic conditions for DPB were used to induce and enrich the target DPB bacteria for 19 days by a gradual transition of enrichment culture. The successful domestication and cultivation of DPB provides a new method to remove nitrogen and phosphorus efficiently and steadily in municipal wastewater.