SBR反应器中全自养硝化颗粒污泥的特性研究

Characteristics of Autotrophic Nitrifying Granular Sludge in Sequencing Batch Reactor

在SBR反应器中接种硝化污泥,研究了污泥颗粒化过程中某些性能的变化,包括沉降性能、胞外聚合物、氧利用速率等.结果表明,当SBR沉淀时间由39min缩短至10min时,污泥SVI由接种时的110mL/g降低至24～42mL/g.EPS(以VSS计)中蛋白质含量由接种时的163mg/g增长到250～270mg/g;而EPS中多糖变化不大,基本在20～30mg/g;硝化颗粒污泥中EPS的蛋白质/多糖的比例基本在9～13左右.反应器中污泥浓度(以VSS计)约2.5g/L,VSS/SS基本保持在85%～90%.在反应器运行初期,污泥中氨氧化菌的活性(SOUR-A)和亚硝酸氧化菌的活性(SOUR-N)不断升高,在第17d,SOUR-A和SOUR-N分别达到259mg/(g·h)和119mg/(g·h);为接种污泥的2.4和5.3倍.当硝化颗粒形成且粒径不断增大后,污泥的SOUR-A和SOUR-N开始降低.污泥中异养菌的活性(SOUR-H)和内源呼吸活性(SOUR-E)在颗粒化过程中都保持在10mg/(g·h).

The variation of some performances of the sludge during granulation process was investigated by seeding the nitrifying sludge in sequencing batch reactor,the sludge volume index （SVI）,extracellular polymeric substances （EPS）,specific oxygen uptake rate （SOUR） and ammonia-oxidizing activity were monitored. The experimental results indicated that when the settling time was gradually decreased from 39 min to 10 min,the SVI decreased from the initial 110 mL/g to 24-42 mL/g. The protein content in EPS increased from 163 mg/g to 250-270 mg/g,while polysaccharide content in EPS almost remained around 20-30 mg/g. The ratio of protein/ polysaccharide in EPS was about 9-13. The sludge concentration in SBR was 2. 5 g/L and the ratio of VSS/S was 85% -90% . At the initial phase of granulation process,the ammonia-oxidizing activity （SOUR-A） and the nitrite-oxidizing activity （SOUR-N） gradually increased and reached 259 mg/（g·h） and 119 mg/（g·h） at day 17,respectively,they were 2. 4 times and 5. 3 time of the seeding sludge. The SOUR-A and SOUR-N began to decrease when the granular size increased. The activity of heterotrophic microorganisms （SOUR-H） and the endogenous respiration activity （SOUR-E） was kept at 10 mg/（g·h） during the granulation process.