摘要
目的:运用HPLC法建立三七丹参颗粒中的人参皂苷Rg1及Rb1含量测定方法。方法:采用ZORBAXNH2(4.6nm×150nm)流动相为乙腈:0.1%H3PO4(82:18);流速为0.8mm·min-1,检测波长为203nm。结果:该法回收率为人参Rg1为99.73%,RSD为0.6%,人参Rb1为99.52%RSD为0.4%。结论:本法操作简单,准确,为测定三七丹参颗粒中人参皂苷Rg1和Rb1的含量提供了可靠的方法。
Objection:To establish a method for determination of GensenosideRg1 and Rb1 in Sanqidanshen Granule by HPLC Methods: The determination was carried out with ZORBAXNH2 column (4. 6nm × 150nm) using methanol-water (82:18)as thw mobile plase at a flow rate of 0. 8ml/min and detected at the wave longth 203nm . Result:The recovery of this method in GensenosideRg1 was 99. 37% RSD was 0. 6% ;in GensenosideRb1 was 99. 52% RSD was 0. 40% . Conclusion:The method was simple and accurate and could bs used to Determination of GensenosideRg1 and Rb1in Sanqidanshen keli .
出处
《中医药学报》
CAS
2010年第2期108-109,共2页
Acta Chinese Medicine and Pharmacology
基金
黑龙江省科技攻关项目(编号:GC07C6020214)