Twist基因在非小细胞肺癌中的表达及意义

Expression and significance of Twist gene in non-small cell lung cancer

目的:探讨转录调节因子Twist在非小细胞肺癌(NSCLC)组织中的表达及临床意义。方法:应用兔抗人Twist多克隆抗体采用SP免疫组织化学方法检测45例NSCLC组织及12例癌旁正常肺组织中Twist蛋白表达情况。结果:在45例肺癌组织标本中,Twist基因阳性表达为31例(68.9%),癌旁组织中为2例(16.7%),两者比较差异有统计学意义(χ2=10.599,P<0.01)。Twist高表达与肺癌分化程度相关(P<0.05)。结论:NSCLC组织中存在Twist基因的高表达,Twist基因表达的上调,对肿瘤的发生、发展起着重要作用。

Objective：To investigate the expression and clinical significance of Twist protein in non--small cell lung cancer （NSCLC）. Methods：The Twist expression was detected by immunohistochemical SP method in 45 eases of NSCLC and 12 cases of tumor adjacent normal lung tissue. Results.The positive rate of Twist gene was 68.9% ,accounting for 31 eases of 45 cases of lung cancer specimens, while the positive rate of the tumor adjacent normal lung tissue was 16.7% ,accounting for 2 of the 12 eases, with a significant difference between the two （Х^2= 10. 599,P 〈 0. 01）. The high Twist expression level was positively correlated with lung cancer differentiation （P 〈 0. 05）. Conclusion：The high expression of Twist gene is found in NSCLC. The Twist expression in NSCLC is up--regulated, which play a vital role in progression of tumor.