胡黄连苦苷Ⅱ对大鼠肝纤维化的治疗作用

Therapeutic effect of Picroside Ⅱ on liver fibrosis in rats

目的研究胡黄连苦苷Ⅱ对CCl4致大鼠肝纤维化的治疗作用。方法56只大鼠分为正常对照组、模型组、胡黄连苦苷Ⅱ治疗组和胡黄连苦苷Ⅱ对照组,采用CCl4腹腔注射诱导大鼠肝纤维化模型,造模第8周,检测大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(ALB)、透明质酸(HA)、层粘连蛋白(LN)、Ⅳ型胶原(Ⅳ-C)、Ⅲ型前胶原(PCⅢ)水平。结果与正常对照组相比,模型组血清ALT、AST、HA、LN、Ⅳ-C、PCⅢ水平显著增高,血清ALB水平显著降低。经胡黄连苦苷Ⅱ干预后,上述指标均显著改善(P<0.01),与正常对照相比较仍有显著性差异(P<0.05)。胡黄连苦苷Ⅱ对照组上述各指标与正常对照组无明显差异(P>0.05)。结论胡黄连苦苷Ⅱ有保护肝细胞、预防CCl4诱导的大鼠肝纤维化作用。

Objective It is to investigate the therapeutic effect of Picroside Ⅱ on liver fibrosis induced by carbon tetrachloride（CCl4） in rats.Methods Fifty-six rats were randomly divided into 4 groups： normal control group,model group,Picroside Ⅱ treatment group and Picroside Ⅱ control group.The rat liver fibrosis model was induced by CCl4 administration.At the end of the 8th week,serum alanine aminotransferase（ALT）,aspartate amino transferase（AST）,albumn（ALB）,hyaluronic acid（HA）,laminin（LN）,collagen IV（IV-C） and type III procollagen（PCIII） were detected.Results Compared with the normal control group,the levels of serum ALT,AST,HA,LN,IV-C and PCIII were significantly increased,and the level of serum ALB significantly was decreased in the model group.After the treatment of Picroside Ⅱ,all the above-mentioned indices were significantly improved（P0.01）,and there were significant differences between the Picroside Ⅱ treatment group and the normal control group（P0.05）.There was no significant difference in all the above-mentioned indices between the Picroside Ⅱ control group and the normal control group（P0.05）.Conclusion Picroside Ⅱ can improve hepatocyte function and inhibit the formation of liver fibrosis induced by CCl4.