缝隙连接蛋白43磷酸化在兔脑血管痉挛中的表达变化

Expression changes of connexin 43 phosphorylation on cerebral vasospasm in rabbits

目的研究缝隙连接蛋白43(Cx43)磷酸化在兔2次蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)中的表达变化。方法新西兰大白兔72只,随机分为:正常对照组(18只)、SAH组(又分3、7、14 d 3个亚组,每个亚组各18只)。采用枕大池2次注血法建立兔SAH后CVS模型。脑血管造影及光镜观察分析基底动脉的直径及形态学变化,应用Western blotting检测Cx43蛋白磷酸化表达的变化。结果(1)在SAH后第7天,脑血管造影及光镜观察证实基底动脉管腔狭窄程度与形态学变化最明显,第14天逐渐缓解,但与正常对照组相比仍有统计学意义;(2)Western blotting结果显示,SAH后磷酸化的Cx43(P-Cx43)蛋白表达以第7天表达最高,14 d开始下降,但仍高于正常对照组;去磷酸化的Cx43(NP-Cx43)蛋白表达以第7天表达最低,14 d开始升高,但仍低于正常对照组。结论SAH后,基底动脉Cx43发生磷酸化,其表达水平的变化与CVS发生发展的时相性一致,提示Cx43磷酸化可能在CVS的发生发展过程中起作用。

Objective To investigate the expression changes of connexin 43（Cx43） phosphorylation in the pathogenesis of cerebral vasospasm（CVS） after experimental subarachnoid hemorrhage（SAH）.Methods 72 New Zealand pure rabbits were afforded.Models of CVS after SAH were successfully made via double blood injection into the cisterna magna.Angiography and hematoxylin and eosin staining method were respectively performed to observe the diameter and morphological changes of the basilar artery.Western blotting was employed to determine the expression changes of Cx43 phosphorylation in basilar artery.Results On 7 d after SAH,arterial narrowing and morphological changes in vascular structure were significantly more than those in other groups,and the changes were relieved gradually on 14 d after SAH;Western blotting result indicated that the expression of phosphorylated Cx43 protein on 7 d was greater than that in other groups,and it was decreased on 14 d after SAH,but it was still significantly higher than that in control group.And the expression changes of NP-Cx43 protein made a contrast with P-Cx43.Conclusion There is a coincidence between the expression changes of Cx43 phosphorylation and CVS after SAH in rabbits,implying that Cx43 phosphorylation may play an important function in the happening and progress of CVS.