束缚应激对卒中后大鼠的影响及帕罗西汀的干预作用

Effect of restraint stress on poststroke rats and effect of paroxetine hydrochloride

目的探讨束缚应激对脑卒中后大鼠行为的影响及帕罗西汀的干预作用及其可能机制。方法选择雄性SD大鼠,将opendfield评分相近的大鼠随机分为对照组、卒中组、束缚组、卒中后束缚(PSR)组和药物治疗组,观察各组大鼠不同阶段的行为改变,测定大鼠下丘脑5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)神经递质含量和含量脑内c-fos表达。结果与对照组比较,PSR组大鼠下丘脑5-HT神经递质明显降低,下丘脑室旁核c-fos蛋白的表达增加;与卒中后束缚组大鼠比较,药物干预组糖水消耗量增加、Open-Field测定水平得分和直立得分增加,脑内5-HT含量升高(P<0.01),下丘脑室旁核c-fos蛋白的表达减少。结论施加束缚应激后脑卒中大鼠出现了明显的抑郁行为,大鼠脑内单胺递质含量降低,下丘脑室旁核c-fos基因表达增加;盐酸帕罗西汀可改善大鼠抑郁行为,其抗抑郁作用可能与提高脑内5-HT含量和下调下丘脑室旁核c-fos基因表达有关。

Objective To explore the effect of restraint stress on poststroke rats and the effect and mechanism of paroxetine hydrochloride.Methods 75 male Sprague Dawley rats were randomly divided into control group,stroke group,restraint group,poststroke restraint（PSR） group and drug treatment group after the measurement of Open-Field test.Rats in each group were processed according to molding.After that the behavioral evaluation was done with consumption of sucrose water,Open-Field test,and then these rats was sacrificed and the brain was taken.The contents of the monoamine neurotransmitter of 5-HT,NE,DA in the brain and expression of c-fos in the the hypothalamic paraventricular nucleus（PVN）,and the effects of paroxetine hydrochloride interference in the PSR model rats were obsevered.Results The consumption of sucrose water,according to the scores of Open-Field test upright and horizonal activities,the contents of the monoamine neurotransmitter,were lower than those in the control group.The expression of c-fos in PVN decreased,compared with the control group.The consumption of sucrose,the scores of horizontal and upright activity of Open-Field test,the 5-HT content were increased significantly in the drug treatment group.The expression of c-fos in PVN decreased,compared with the PSR group.Conclusion After restraint stress was performed,the poststroke rats developed significantly depressive behavior.The content of monoamine neurotransmitter was lower and the expression of c-fos in PVN was increased in the poststroke restraint stress model rats.The molecular mechanism of paroxetine relieving the symptoms of the poststroke restraint stress rats disorders may be related with the increase contents of the monoamine neurotransmitter in brain and the decrease expression of c-fos in PVN.