β-榄香烯抑制人骨髓瘤细胞RPMI-8226增殖的实验研究

Effect of β-elemene on proliferation of human multiple myeloma cells RPMI-8226

目的研究β-榄香烯对人骨髓瘤RPMI-8226细胞增殖、周期和凋亡的影响。方法用噻唑蓝(MTT)法检测β-榄香烯对骨髓瘤RPMI-8226细胞增殖的影响;Annexin V/PI双标流式术检测β-榄香烯对骨髓瘤RPMI-8226细胞周期、细胞凋亡的影响;Hoechst33342/PI双染荧光显微镜观察β-榄香烯处理后骨髓瘤RPMI-8226细胞形态学变化。结果β-榄香烯对RPMI-8226骨髓瘤细胞生长具有显著抑制作用,呈浓度和时间依赖性。β-榄香烯处理后,与对照组相比G0/G1细胞比例显著升高,而S、G2/M细胞比例降低。β-榄香烯作用48 h可诱导骨髓瘤细胞凋亡,凋亡率随药物浓度而递增。结论β-榄香烯可有效抑制人骨髓瘤细胞增殖;其抑制作用与细胞周期阻滞和细胞凋亡激活有关。

Objective To investigate the effects of β-elemene on proliferation,cell cycle and apoptosis of human multiple myeloma RPMI-8226 cells.Methods The effect of β-elemene on the growth of human multiple myeloma RPMI-8226 cells was studied through MTT assay.The effects of β-elemene on the cell cycle and apoptosis of RPMI-8226 cells was studied by combined Annexin-V protein iodide staining.The morphological changes of RPMI-8226 cells was studied by combined Hoechst33342-PI staining after treatment with β-elemene.Results β-elemene inhibited the proliferation of RPMI-8226 cells in a time-and dose-dependent manner.Compared with the control cells,after the treatment with β-elemene,the proportions of the RPMI-8226 cells in the G0/G1 phase increased,and the proportions of the RPMI-8226 cells in the S and G2/M phases decreased.Treatment with β-elemene for 48 h induced apoptosis of RPMI-8226 cells in a dose-dependent manner.A lot of the typical cells with apoptosis were seen by combined Hoechst33342-PI staining after treatment with β-elemene.Conclusion β-elemene is able to inhibit the proliferation of RPMI-8226 cells by arresting the cell cycle and inducing the cell apoptosis.