Identifiler体系在276例单亲亲权鉴定中的应用分析

Usefulness of Identifiler system in 276 cases of duo paternity testing

目的探讨Identifiler体系在单亲亲权鉴定案例中的应用价值。方法采用荧光标记STR-PCR复合扩增、毛细管电泳基因分型技术,应用3100 Avant遗传分析仪对276例单亲亲权鉴定的个体进行15个短串联重复串联序列(STR)基因座的基因分型。结果在276例的单亲亲权鉴定中,认定亲权关系199例,占72.10%,其中亲权指数(CPI)≥2 000,父权相对机会(RCP)≥99.95%有183例,13例CPI<2 000,3例出现一个基因座单步突变;排除亲权关系77例,占27.90%,其中2例只出现1～2个不符合遗传规律基因座,通过加测母亲样本,排除指标均大于3个。结论应用Identifiler体系的15个STR基因座对常规单亲亲权案例能成功地开展鉴定分析工作,但当CPI<2 000或有1～2不符合遗传规律基因座时,须增加检测基因座和加测母亲样本,以提高亲权指数或增加排除指标,规避可能的错误鉴定结论。

Objective To analyze the application value of Identifiler kit in duo paternity testing.Methods The technique of fluorescent dye labeling multiplex STR-PCR and capillary electrophoresis and DNA sequencer GeneScan.Genetyping of 15 short tandem repeat（STR） were adopted in genetyping a sample of individuals in 276 cases parentage testing by using 3100 Avant.Results Among 276 cases of duo parentage testing,199 cases of determined paternal relation were 72.10% in all cases,and cumulative paternity index（CPI） was 2 000 or more than 2 000.In 183 cases,the relative chance of paternity（RCP） was 99.95% or more than 99.95%.And CPI of 13 case was small than 2 000,3 cases had a step mutation of a gene locus.77 cases were excluded paternity relationship.The percentage was 27.9% in all cases.2 cases had one to two loci incoordinated genetic law in these exclusive cases,then exclusive indexes were more than three items by additional detecting mother′s sample.Conclusion Using Identifiler system of 15 STR loci can successfully analyze duo paternity testing.But worth of watching,when CPI is small than 2 000 or having 1-2 incoordinate gene loci,we must add to determine more loci or sample of mother for improving CPI or exclusive index,and then avoiding error identification conclusion.