摘要
以欧亚旋覆花(Inulabritannica L.)无菌苗为材料,研究了不同浓度的噻重氮苯基脲(Thidiazuron,TDZ)对叶片、根愈伤组织诱导的影响。结果显示,在不添加TDZ时,叶片的愈伤组织诱导率为82.9%,且诱导出的愈伤组织生长情况较好。当TDZ的浓度为0.05mg/L时,欧亚旋覆花根的出愈率最高,达100%,且愈伤组织生长佳、有光泽。
The effect of different concentrations of Thidiazuron （TDZ） on callus induction of the leaves and roots of Inula britannica was studied using sterile seedling as explants. The results showed that the leaves on MS without TDZ had a higher induction rate, and the calli grew better. The medium containing TDZ 0.05 mg / L was proved to be the most effective for root inducing callus and the induction rate was 100%, the callis grew better and had glossy.
出处
《湖北农业科学》
北大核心
2010年第4期798-799,共2页
Hubei Agricultural Sciences
