摘要
为了明确辽宁省根结线虫种类及分布,从辽宁省8个市县区大型蔬菜生产基地的温室中以及露地上采集根结线虫样本82份,对雌虫、雄虫和二龄幼虫的形态学进行观察和测量,制作雌虫会阴花纹切片进行比较鉴定并且应用同工酶技术进行鉴定。结果表明,辽宁省根结线虫发生的种类为南方根结线虫(Meloidogyne incognita)、北方根结线虫(M.hapla)。其中南方根结线虫是危害辽宁省大棚蔬菜的优势种群,北方根结线虫是危害露地作物的优势种群。
In order to distinguish the species of root-knot nematode occurring in Liaoning province, eighty-two samples of root-knot nematode were collected in the greenhouse,which planted with various vegetables and outdoor plants from eight counties of Liaoning. Root-knot nematodes were identified based on morphological characteristics of female,male and second stage juvenile. Moreover,perineal patterns of female were prepared for the identification. Isozyme analysis were used as identification. The results showed that the species occurred in Liaoning include Meloidogyne incognita, M. hapla, M. incognita was prevailing on the vegetables in greenhouse while M. hapla was prevailing on outdoor plants in Liaoning.
出处
《湖北农业科学》
北大核心
2010年第4期865-868,共4页
Hubei Agricultural Sciences
基金
公益性行业科研项目(nyhyzx07-050-4-01)资助
关键词
根结线虫
会阴花纹
同工酶
辽宁省
鉴定
root-knot nematode
perineal pattern
isozymes
Liaoning province
identification
