大鼠间歇训练对海马c-fos蛋白表达的影响

Study of the Influence of Intermittent Exercise on the Expression of c-fos Protein in Hippocampus of Rat

为了观察大鼠间歇训练对海马c-fos蛋白表达的影响。将雄性SD大鼠30只,随机分为对照组(5只)、间歇训练组(25只)。建立大鼠间歇游泳训练模型,采用ABC免疫组织化学法,观察大鼠海马c-fos阳性神经元细胞表达的变化(即c-fos蛋白表达变化),并用图像分析系统和统计学软件进行图像和数据分析。结果表明:训练后大鼠海马不同区域c-fos阳性细胞表达密度明显增高,与对照组相比均有显著性差异(P<0.01);CA2区c-fos阳性细胞表达迅速而明显,训练后0.5 h即达峰值,而后回落;CA3区c-fos阳性细胞表达最为密集,训练后1 h达到高峰,而后快速回落;CA1区c-fos阳性细胞表达相对较少,1 h达到高峰,而后缓慢下降。结论:大鼠间歇训练对海马不同区域c-fos蛋白表达有影响,具有时效性;海马不同区域c-fos蛋白表达在密度、时相上存在明显差异,这可能与海马不同区域对缺血、缺氧的耐受能力有关。

This is to study the effect of intermittent exercise on the expression of c-fos protein in hippocampus. Male SD rats were randomly divided into two groups：control group and intermittent exercise group. To establish the intermittent swimming exercise models in rat,and detect the change of the expression of c-fos positive cells in hippocampus by using ABC immunohis- tochemistry means,then analyze the image and data by imaging analyzing system and statistic software. The result shows that： after exercise, the density of c-fos positive cells were significantly higher in the different regions of hippocampus, with significant difference as compared with the control group（P 〈0. 01 ） ;in CA2,the expression of c-los positive cells were increased obviously, gets up to the top at 05h, and then descend;In CA3, the express density of c-fos positive cells is the highest, the top at lh,and then descend rapidly;in CA1 ,the express density of c-fos positive cells is small relatively,the top at lh,and then descend slowly. Conclusion ： intermittent exercise could affect the expression of c-fos protein in hippocampus, and the expression of c-fos protein was timelines ; the express density and phase of c-fos positive cells in the different regions of hippocampus have significant difference due to the tolerance of ischemia and anoxia.