摘要
为提高犬的精液冷冻效果,在冷冻保存液配方中添加不同浓度糖(葡萄糖、果糖),将平衡时间和熏蒸时间做系统对比。经系列分组试验发现,含0.5%果糖组中精子活力显著高于其他组别(P<0.05);平衡时间120min(P<0.05);熏蒸时间20min冷冻效果较好。结果表明犬精子冷冻液添加低浓度果糖优于葡萄糖,而且平衡时间为120min,熏蒸时间为20min可得到稳定的冷冻效果。
The study was conducted to cryopreserve canine semen for optimizing cryopreservation medium. The different concentration of sugar （Glucose Fructose）, different balance times, different fumigation times were selected systematically in the cryopreservation medium. The results showed addition of 0.5% fructose was better than glucose at any concentration （P 0.05）. 120 min balance time and 20 min liquid nitrogen fumigation time were excellent for motility of frozen sperm （P 0.05）. In conclusion, the optimized procedures （0.5% fructose, 120 min balance time and 20 min） were feasible and steady for freezing canine sperm.
出处
《中国农学通报》
CSCD
北大核心
2010年第10期14-16,共3页
Chinese Agricultural Science Bulletin
基金
河南农业大学博士基金"小动物精液冷冻技术研究与应用"(30300092)
关键词
犬
精子
葡萄糖
果糖
冷冻保存
canine
sperm
glucose
fructose
cryopreservation
