用SSR标记法对黑龙江省稻瘟菌无毒基因的检测

Identification of Magnaporthe Oryzae Avirulence Gene in Heilongjiang Province with SSR Marker

研究黑龙江省稻瘟病菌无毒基因的分布情况,使用与无毒基因Avr-pit、Avr-pia、PRE1紧密连锁的3个SSR标记对黑龙江省不同地区的195个稻瘟菌菌株的DNA进行PCR扩增;3个无毒基因在黑龙江省的出现频率有明显差异,无毒基因Avr-pit的出现频率为43.59%,无毒基因Avr-pia出现频率36.92%,无毒基因PRE1的出现频率为30.26%。黑龙江省稻瘟病菌的群体结构复杂,不同地区之间各无毒基因出现频率存在较大的差异,同一基因在不同地区分布差异也较大;研究为稻瘟病菌的分子标记、遗传多样性研究和分子育种奠定了基础。

The aim was to study the distribution of Magnaporthe oryzae avirulence gene in Heilongjiang Province. PCR amplification of DNA in 195 Magnaporthe oryzae strains of different region Heilongjiang province by three SSR markers which were closely linked to the avirulence gene Avr-pit, Avr-pia and PRE1. The frequency of different avirulence gene was significant different in Heilongjiang province, and the appearance frequency of these three avirulence genes Avr-pit, Avr-pia and PRE1 were 43.59%, 36.92% and 30.26%. The group structure ofMagnaporthe oryzae was complex in Heilongjiang Province. The distribution of avirulence gene varied in different regions even in the same region. The research laid the foundation for the molecular marker and genetic diversity ofMagnaporthe oryzae and molecular breeding.