水稻干燥组织DNA模板的快速制备

Rapid Extraction of Rice Genomic DNA from Natural Dried Tissue

用碱处理水稻干燥组织,浸出液直接用作PCR扩增的模板,扩增结果稳定、准确,与CTAB法和SDS法提取的DNA扩增效果相比没有显著差异。用此法提取水稻自然干燥的叶片、叶鞘、茎杆、颖壳和根DNA,质量良好,能够满足SSR分析要求。

After the alkali treatment for rice natural dried tissues, taking the alkaline leaching solution directly as template for PCR amplification, the amplification results are stable, accurate, and are insignificantly different in comparison with the methods CTAB and SDS. The DNA extracted from natural dried rice flag leaf, leaf sheath, culm, glume husk and roots, have good quality, and can meet with the SSR analysis.