花椰菜RAPD随机引物的筛选

Screening Random Primers for RAPD Analysis in Cauliflower Cultivars

为了了解37份花椰菜种质资源的遗传多样性和亲缘关系,为花椰菜育种提供理论基础,以其中7份来自不同产地的花椰菜品种为模板对80条随机引物进行了初步筛选。48条随机引物能获得多态性扩增,25条引物的RAPD分析结果无多态性,7条引物的未获得扩增条带;引物S11、S15、S16、S17、S18和S65扩增条带比较稳定、清晰且具有多态性,多态性比例在16.7%～75.0%之间,可作为花椰菜RAPD分析的随机引物。

To elucidate the genetic diversity and phylogenetic relationships of 37 cauliflower cultivars, provide a theoretical basis for cauliflower breeding, 80 random primers were carried out a preliminary screening with 7 among the cauliflower cuhivars as amplification template. Among which 48 primers gave polymorphism, 25 primers gave monomorphic and 7 primers without amplified bands within the varieties. Stable, clear and polymorphic bands could be obtained with primers Sll, S15, S16, S17, S18 and S65, the percentage of polymorphie bands ranging from 16.7% to 75.0%, and these primers appear to be useful for genetic diversity studies in cauliflower eultivars.