摘要
从中国海南和云南橡胶主产区分离到22株橡胶炭疽病菌,生物学特性和形态学观察表明其中存在尖孢炭疽菌,ITS序列分析进一步证明侵染中国海南和云南橡胶的炭疽菌为胶孢炭疽和尖孢炭疽。ITS序列多样性分析表明,15株尖孢炭疽菌之间遗传差异较小,7株胶孢炭疽菌之间差异较大。
The internal transcribed spacer regions （5.8S/ITS） of the ribosomal RNA gene from 22 isolates of colletotrichum leaf disease of rubber were amplified through the polymerase chain reaction with the universal primers and sequenced. And their biological characters also tested. The result revealed that only 7 isolates belong to Colletotrichum gloeosporioides. The remaining 15 isolates represented Colletotricbum acutatum.Intraspecific genetic variation of Colletotrichum acutatum isolated from rubber was relatively low, which was found through rDNA-ITS sequence analyses. However, intraspecific genetic variation of Colletotriehumgloeosporioides was relatively abundant.
出处
《中国农学通报》
CSCD
北大核心
2010年第12期221-226,共6页
Chinese Agricultural Science Bulletin
基金
国家科技支撑计划课题"热带特色经济作物病虫害防控技术研究与示范"(2007BAD48B06)
