抗腐霉利核盘菌生物学及生理生化特性研究

Biological,Physiological and Biochemical Characteristics of Procymidone-resistant Sclerotinia sclerotiorum

探讨了抗腐霉利的核盘菌突变抗性株与亲本敏感株的生物学及生理生化特性,为研究核盘菌对腐霉利产生抗药性的生理机制提供参考;在不同的营养、酸碱度、温度条件下测定了突变菌株的生物学特性,并用生物法测定了其渗透压、电导率和PLA活性;突变株分别以在乳糖为碳源和缺氮处理的培养基中以及pH为6～7时生长最快,而亲本株在乳糖为碳源和蛋白胨为氮源的培养基上以及pH值为5时生长最快。在相同的渗透压条件(葡萄糖浓度)下,突变株对渗透压较亲本株更为敏感。亲本株对低渗透压(1%)表现敏感,突变株对高渗透压(>4%时)表现敏感。在相同处理时间内,突变株电导率均高于亲本株,并随着处理的延长,二者的电导率均升高。不同浓度腐霉利处理或饥饿处理时,突变株与亲本株PAL活性均增加,但突变株PAL活性始终高于亲本株;突变株的渗透压、电导率和PAL活性变化均明显于敏感株,核盘菌抗药性与其渗透调节、电解质渗透和相关酶活性具有一定的相关性。

Biological, physiological and biochemical characteristics were studied between procymidone-resistant mutants and their parent sensitive strains of Sclerotinia selerotiorum. It provided references on resistance mechanism of S.sclerotiorum to procymidone; At the different conditions of Nutrition, pH and temperature, biological characteristics were tested, and their osmotic pressure, conductivity and activity of PLA （phenlalanine ammona-lyase） were also tested by biological methods; The mutants and parent strains grew fastest respectively when carbon-nitrogen source and pH value were lactose, non-nitrogen, 6-7 and lactose, peptone and 5. Both grew similar in different temperatures. On the same osmotic stress （dextrose concentration）, the mutants had more sensitive to osmotic stress than parent strains. The parent strains and mutants respectively were sensitive to low （1%） and high （〉 4%） osmotic stress. Relative conductivity of the mutants was always higher than that of parent strains in same treat time. Relative conductivity of both rose with extension of treat time. Respectively treated with hunger and procymidone in different concentrations, the activity of PALof both increased, but the mutant had higher PAL activity than its present strain. Osmotic pressure, conductivity and PAL activity of the mutants were more significant than those of present strains. Resistance of S. sclerotiorum was related to its osmotic regulation, electrolyte leakage and correlate enzyme a.ctivity.