响应面法优化槟榔中多酚提取工艺条件的研究

Optimization of Condiations to Extract Polyphenol of Arecanut Using Response Surface Methodology

采用响应曲面法,建立了温度,甲醇浓度,料液比,对槟榔多酚提取率影响的二次多项回归模型。验证了模型的有效性。响应曲面分析表明,随着温度和甲醇浓度的增加,槟榔多酚提取率呈先上升,后下降趋势,随着温度和料液比的增加槟榔多酚提取率逐渐增加。随着甲醇浓度和料液比的增加,槟榔多酚提取率也是呈先上升后下降的趋势,但二者交互作用不显著,提取温度是影响多酚提取率的主要因素,其次为料液比和甲醇浓度。综合优化得出提取温度为65.7℃,甲醇浓度为62%,料液比为1︰23.5g/mL,此时槟榔多酚提取率为11.23mg/g.

Response surface methodology（RSM） was employed and second order quadratic equation for the temperature, methanol concentration, ratio of material to liquid of extracting polyphenol of arecanut was built and the applicability of the model equation was verified. The result of the response surface showed that with the increasing of temperature and methanol concentration, the extraction efficiency of arecanut polyphenol increased at first but decreased later, with the increasing of temperature and ratio of material to liquid the extraction efficiency of arecanut polyphenol increased gradually, while with the increasing of methanol concentration and ratio of material to liquid the extraction efficiency of arecanut polyphenol increased at first but decreased later as well but the interaction of both was not significant. The main factor which effects the extraction efficiency of arecanut polyphenol is temperature then is the ratio of material to liquid and methanol concentration. The optimum process parameters for extracting the polyphenol of arecanut were obtained as： temperature 65.7℃, methanol concentration 62%, ratio of material to liquid 1：23.5 g/mL. At this time the extraction efficiency of arecanut polyphenol was 11.23 mg/g.