脉平对离体内皮细胞凋亡及凋亡调控基因的影响

Effects of Maiping on apoptosis and apoptosis genes in vascular endothelial cells

目的探讨血管紧张素Ⅱ在不同浓度和不同作用时间下对内皮细胞凋亡率及凋亡调控基因的作用及脉平对其影响。方法采用流式细胞仪测定在血管紧张素Ⅱ不同浓度和不同作用时间下内皮细胞凋亡率及凋亡基因Fas和Bcl-2表达的变化和应用脉平后对其的影响。结果血管紧张素Ⅱ可明显促进凋亡率及凋亡基因的表达,且其作用呈剂量依赖性和时间依赖性;经脉平处理后的内皮细胞对AngⅡ的反应完全不同,凋亡率明显降低,同时Fas的表达量随药物浓度增加和作用时间延长而降低,Bcl-2则增高。结论血管紧张素Ⅱ具有明显的促进细胞凋亡和凋亡基因表达的作用,脉平对细胞凋亡和凋亡基因表达有一定的调节作用。

Objective To study the effects of angiotensin II（AngII） in different concentrations and different action time on the ratio of apoptosis and expression of apoptosis genes in vascular endothelial cells and the influences of maiping on it.Methods Flow cytometer was used to measure the apoptosis rate and the expression of apoptosis genes（Fas and Bcl-2） induced by AngII in different concentrations and different action time,and influenced by the drug.Results The apoptosis rate and the expression of apoptosis genes were induced and increased by AngII with the increase of concentrations and action time compared with the control.The expression of Fas was decreased and Bcl-2 increased with the increase of the concentrations and action time of AngII after the uses of Maiping compared with AngII group.Conclusion The apoptosis rate and the expression of apoptosis genes may be induced and increased by angiotensin II with the increase of the concentrations and the action time.The use of Maiping may have the regulating effect on the apoptosis rate and the expression of apoptosis genes in vascular endothelial cells.