不同化学染色法对日本血吸虫虫卵染色的效果分析

Analysis of the effectiveness of different chemical dyes at staining Schistosoma japonicum eggs

目的探索不同化学染色法对日本血吸虫虫卵染色的效果,以期获得在镜检下可分辨虫卵和粪渣的染色方法,为粪检血吸虫虫卵的计算机自动化识别奠定基础。方法用AO-EB、罗丹明、中性红、酸性品红、甲苯胺兰、台盼蓝、考马斯亮蓝和孔雀绿等化学染料,选择3个不同浓度和2个不同温度条件,分别对含日本血吸虫虫卵粪液进行染色、涂片镜检,用虫卵和粪渣反差程度作为主要评价指标,比较各种方法的染色效果。结果在8种化学染色方法中,以AO-EB染色和中性红染色的虫卵与粪渣其反差最大,镜下粪涂片中虫卵易于辨认,AO-EB法和中性红法的检出率分别为67.5%和65.0%,明显高于改良加藤法的检出率(40.0%)。结论AO-EB法和中性红染色法有助于提高粪检血吸虫虫卵检出率。

Objective To examine the effectiveness of different chemical dyes at staining Schistosoma japonicum eggs in order to provide a method of staining that can easily distinguish eggs and fecal residue under a microscope.This may have considerable significance for the automated identification of schistosome eggs.Methods At different concentrations and temperatures,S.japonicum eggs in liquid feces were stained with the following chemical dyes：AO-EB,rhodamine,neutral red,acid fuchsin,toluidine blue,trypan blue,Coomassie brilliant blue,and malachite green.Then,the effects of various staining methods were compared with smear microscopy of eggs and fecal residue to provide a primary index to assess contrast.Results Of the eight staining methods,AO-EB staining and neutral red staining resulted in the greatest difference between eggs and fecal residue.These two methods allowed eggs to easily be identified under the microscope.The detection rate for AO-EB and neutral red staining was 67.5% and 65.0%,respectively.These methods were clearly superior to modified Kato staining,which had a detection rate of 40.0%.Conclusion AO-EB staining and neutral red staining helped to increase the rate of schistosome egg detection.