石斛提取的丁香酸对氧化条件下人类晶状体上皮细胞增殖的影响

The effect of syringic acid extracted from dendrobium on human lens epithelial cells in oxidation condition

目的探讨石斛提取的单体丁香酸对氧化条件下人类晶状体上皮细胞(human lens epithelial cell,HLEC)的形态及生长的影响。方法取对数生长期的HLEC,分为正常对照组(HLEC+DMEM)、阳性对照组(正常对照组+500μmol/L H2O2)、丁香酸高剂量组(阳性对照组+0.4 g/L丁香酸)、丁香酸中剂量组(阳性对照组+0.2 g/L丁香酸)、丁香酸低剂量组(阳性对照组+0.1 g/L丁香酸),在倒置显微镜下观察HLEC的形态变化,MMT法检测丁香酸对HLEC增值的影响。结果在H2O2的培养条件下,HLEC的死亡率增加,生长受到抑制,而用丁香酸干预的条件下的HLEC则保持了上皮形态,死亡率降低。阳性对照组HLEC的增殖被H2O2抑制,抑制率为52.99%,与其他各组比较差异有显著性,P<0.05;中剂量的丁香酸存活率达到79.97%,与高、低剂量的丁香酸比较差异有显著性,P<0.05。结论丁香酸对氧化损伤的HLEC具有一定的保护作用。

Objective To study the protention to human lens epithelial cells of syringic acid extracted from Dendrobium Noble in oxidation condition. Methods The second generation human lens epithelial cell （HLEC） were cultured in H2O2 and the syringic acid extracted the Dendrobium nobileg of different concentration for 24h. There were divided into 5 groups, which is normal control group （ HLEC ＋ DMEM） , positive control group （normal control group ＋ 500 μmoL/L H2O2 ） , syringic acid high dose group （positive control group ＋ 0.4 g/L syringic acid）, syringic acid medium dose group （positive control ＋0.2 g/L syringie acid）, syringie acid low dose group （positive control ＋ 0.1 g/L syringie acid） , The morphous changes of the cells were then observed when they were intervened in different concentration of syringic acid. The effect of different concentration of syringic acid to the growing of HLEC in H2O2 was determined by MTT assay. Result The HLEC ＇S death rat increased and the growth was inhibited in high H202 ,the rate of inhibition was 52.99% , there was significant difference between the survival rate Compared with the other groups, P 〈 0.05. Few rate HLEC died in high H2O2 which was intervened by syringic acid, their growing was good and the morphous was not destroyed. The HLEC＇s survival rate was 79.97% in medium dose syringic acid, Compared with the group of high and relatively low doses of syringie acid, there was a significant difference, P 〈 0.05. Conclusion The morphous of HLEC were abnormal, the a- mount of cell deereaed in H2O2 ;the morphological changes of HLEC and amount＇s decrease were delayed and prevented after intervened by syringic acid, maintained HLECS inhere characteristics, showing that syringic acid could protect HLEC from being damaged by H2O2.