夏大豆产量相关根系性状及QTL定位

Study on root trait related yield in soybean growing in summer and QTL mapping

以冀中南地区夏播表现相对高产和低产的15个大豆品种为材料,采用盆栽方法,在出苗后41d(开花结荚期)、65d(结荚鼓粒期)分别对根系进行2年及2点取样,测定形态和生理性状;利用"科丰1号×南农1138-2"衍生的RIL群体为材料,对产量相关根系性状进行QTL定位。结果表明,两种产量类型间的根活形态增量均达到极显著差异,且与产量呈极显著正相关,该期的根活形态增量可作为大豆产量相关根系指标。QTL分析检测到4、3、3个QTLs分别控制根活质量增量、根活长增量、根活体积增量,位于N6-C2、N20M、N18-K、N14G、N3B1连锁群上。三性状各有1个贡献率大的QTL,且均位在N6-C2上,其他QTLs效应均较小。

Fifteen summer soybean varieties with relative higher and lower yields in mid - south of Hebei province were used for researching on root traits by pot experiment. At 41, 65 days after seeding, the root morphological and physiological traits were determined during two years and two location respectively. The RIL population derived from Kefeng 1 × Nannong 1138 - 2 was used to mapping the QTLs of root trait related to yield. Results showed that the increment of root- active - morphological traits（ IRRAMT）, namely, increment of the products of root reductive activities and root fresh weights, root total lengths, root volumes, respectively, were significant different between two yield types. The IRRAMT could be used for the index of yield. There identified were fore, three, three QTLs located on N6 - （12, N20M, N18 - K, N14G,N3B1 linkage groups for IRRAW, IRRAL, IRRAV respectively. Each of the traits appeared to have one locus with relatively large effect in comparison with their other loci, and those major ones were located at a same linkage group N6 - C2.