6种云南天然药物对口腔优势菌的抗菌活性测定

Antibacterial activity determination of six kinds of natural herbs in Yunnan on normal oral predominant bacteria in vitro

目的测定6种云南天然药物对口腔临床常见优势菌的体外抗菌活性,从而初步确定其在口腔临床的药用价值。方法采用美国国立实验室标准化委员会推荐的厌氧菌药物敏感试验——琼脂稀释法,测定6种云南天然药物对21株口腔临床常见优势菌模式株、临床分离株的体外抗菌活性,用最小抑菌浓度（MIC）表示。结果6种药物对21株实验细菌均有抗菌作用：对10株致龋菌的MIC值范围：苏木为5~10mg·mL-1;其他5种药物（丽江山慈菇、滇龙胆、诃子、龙血竭、杜仲）均大于20mg·mL-1。对11株牙髓根尖周炎和牙周病的常见优势菌的MIC值范围：苏木均小于等于0.0625mg·mL-1;诃子为10~20mg·mL-1;其他4种实验药物均大于20mg·mL-1。结论6种药物在口腔细菌性疾病的临床治疗中具有潜在的药用价值。

Objective To determine the antibacterial activity of six kinds of natural herbs in Yunnan on normal oral predominant bacteria in vitro. Methods Agar dilution method, the antimicrobial susceptibility testing of anaerobes which was recommended by National Committee for Clinical Laboratory Standard（NCCLs）, was used to determine the antimicrobial susceptibility of herbs to 21 kinds of oral model strains and clinical isolates. The results were expressed by minimum inhibitory concentration（MIC）. Results All six kinds of herbs were effective to the oral predominant bacteria. For the ten kinds of cariogenic bacteria, the MIC of caesalpinia sappan lignum was between 5-10 mg·mL-1, and the MIC of other herbs was above 20 mg·mL-1. For the eleven kinds of predominant bacteria which normally involved in pulp periapical disease and periodontitis, the MIC of caesalpinia sappan lignum was below 0.062 5 mg·mL-1, and the MIC of the terminalia chebula retz was between 10-20 mg·mL-1, and the others were above 20 mg·mL-1. Conclusion The six kinds of herbs in Yunnan could be used in treatment or prevention of oral bacterial diseases.