番鸭呼肠孤病毒诱导的细胞凋亡观察

The Observation of the Apoptosis Induced by Muscovy Duck Reovirus

以番鸭呼肠孤病毒人工感染10日龄健康番鸭,运用原位末端标记技术及免疫组织化学方法,对番鸭呼肠孤病毒感染后番鸭多种组织的细胞凋亡进行检测,对死亡因子FasL的表达进行研究。原位末端标记检测显示,多种组织器官中出现大量阳性细胞,表明番鸭呼肠孤感染可引起肝脏、脾脏、肾脏、肺脏、胸腺、盲肠和法氏囊发生不同程度的细胞凋亡。免疫组织化学研究结果表明,感染番鸭呼肠孤病毒后,肝脏、肾脏、肺脏、胸腺、盲肠和法氏囊等多种组织中可观察到FasL表达,且各种组织FasL表达的强弱与原位末端标记检测的细胞凋亡指数高低相一致。结果表明番鸭呼肠孤病毒诱导细胞凋亡的机制与FasL的表达密切相关。

10-day old Muscovy ducks were infected with Muscovy Duck Reovirus.Apoptosis were detected and the expression of FasL were studied in many infected tissues post infection,using in-situ end labeling technique and immunohisto chemistry method.The result of in-situ end labeling revealed that a large number of positive cells were found in many tissues,which showed that Muscovy duck reovirus could induce varying-degreed apoptosis in the liver,spleen,kidney,lung,thymus,cecum and bursa of fabricius.The result of immunohistochemical study showed that the expression of FasL were observed in liver,kidney,lung,thymus,cecum and bursa of fabricius,and the changes of FasL expression were highly correlated with the level of apoptotic index.The results showed that the mechanism of apoptosis induced by Muscovy duck reovirus is closely related with the expression of FasL.