半胱胺改性叶酸衍生物的制备及其性能研究

Study on preparation and properties of folic acid modified by cysteamine derivatives

在N,N-二环己基碳酰亚胺(DCC)和N-羟基琥珀酰亚胺(NHS)共同催化下制备出半胱胺改性叶酸衍生物,在pH=8的稀氨水溶液中,对纳米金和叶酸衍生物进行偶联。结果表明,叶酸的羧基与半胱胺的巯基发生了反应,叶酸衍生物具有荧光性能,并与纳米金粒子有吸附作用,在叶酸靶向肿瘤诊断治疗方面具有潜在的应用价值。

Folic acid modified by cysteamine derivatives was synthesized through N, N-dicyclohexylcarbon- imide（DCC） and N-hydroxysuccinimide（NHS） as co-catalyst. The folio acid derivative was conjugated with gold nanoparticles in dilute ammonia solution. The results showed that folio acid carboxyl and the --SH of cysteamine had reacted successfully. The products had fluorescent properties. Folio acid deriva- tives adsorbed the nano-Au particles, which might have potential application value in folic acid cancer diagnosis and treatment areas. targeted