乙酰肝素酶、MMP-9在肝素干预的自然流产大鼠模型胎盘组织中的表达及意义

Expression levels and significance of heparitinase and MMP-9 in placental tissues of heparin intervention rat models of spontaneous abortion

目的:比较正常妊娠模型大鼠、自然流产模型大鼠、肝素干预模型大鼠胎盘组织乙酰肝素酶、MMP-9表达特征的差异,探讨两者与自然流产的关系。方法:建立正常妊娠大鼠模型、自然流产大鼠模型和肝素干预大鼠模型,采用免疫组化SABC法测定3组模型孕14天胎盘组织中乙酰肝素酶和MMP-9的表达。结果:与正常妊娠模型组和肝素干预模型组相比,自然流产模型组大鼠胎盘组织中类肝素酶和MMP-9的表达显著升高(P<0.01)。结论:胎盘组织中乙酰肝素酶和MMP-9的表达与自然流产的发生、发展有关,肝素可对自然流产起到一定的预防和治疗作用。

Objective： To compare the expression differences of heparanase and MMP - 9 in rat models of normal pregnancy, spontaneous abortion and heparin intervention spontaneous abortion, explore the relationship between heparitinase, MMP- 9 and spontaneous abortion. Methods： The rat models of normal pregnancy, spontaneous abortion and heparin intervention spontaneous abortion were established. The expression levels of heparanase and MMP - 9 in placental tissues of rat models of 14 gestational clays in the three groups were detected by immunohistoehemieal SABC method. Results： Compared with normal pregnancy group and heparin intervention spontaneous abortion group, the expression levels of heparanase and MMP- 9 in spontaneous abortion group increased significantly （P 〈0. 01 ） . Conclusion： The expression levels of heparanase and MMP- 9 in placental tissues are associated with occurrence and development of spontaneous abortion. Heparin may prevent and treat spontaneous abortion to some extent.