泰拉霉素和红霉素对一氧化氮和前列腺素E2合成抑制作用的比较

In vitro Inhibitory Effects of Tulathromycin and Erythromycin on the Productions of NO and PGE_2

目的以红霉素为对照,探讨泰拉霉素是否具有抗炎作用。方法利用细菌脂多糖(LPS)诱导猪外周血单核细胞(PBMC)过度表达致炎因子而构建的体外炎症模型,采用SYBR Green法实时荧光定量PCR技术,在分子水平研究了不同浓度泰拉霉素和红霉素对一氧化氮(NO)和前列腺素E2(PGE2)合成的影响。结果20μg·mL-1和10μg·mL-1浓度的泰拉霉素与20、10和5μg·mL-1浓度的红霉素均能显著抑制猪PBMC细胞合成NO和PGE2,并抑制诱导型一氧化氮合成酶(iNOS)和环氧合酶-2(COX-2)基因的转录;而5μg·mL-1的泰拉霉素无明显的这种调节作用。结论泰拉霉素和红霉素能在转录和翻译水平通过调节致炎因子的合成而发挥抗炎作用。

Objective The anti-inflammatory effects of tulathromycin were studied. Method In vitro, we make use of the model that is LPS-induced porcine peripheral blood mononuclear cells （PBMC） overexpressing pro-inflammation factors was used, and then the real-time Q-PCR was employed to study the effect of different concentrations of tulathromycin and erythromycin on the generation of NO and PGE2 involved in the inflammatory process on the molecular level. Result Results showed that tulathromycin at the concentrations of 5, 10 and 20 μg·mL-1 significantly decreased the production of NO and PGE2, a similar pattern was also observed on the cyclooxygenase （COX-2） and inducible nitric oxide synthase （iNOS） gene transcription. However, all 5, 10 and 20 μg·mL-1 of erythromycin significantly decreased the production of NO and PGE2 on the transcription and expression level. Conclusion These results support the opinion that macrolides may exert anti-inflammatory effect through modulating the synthesis of NO and PGE2 in inflammatory process.