七味白术散对人类轮状病毒感染乳鼠小肠黏膜上皮细胞的保护作用

Protective Effect of Qiweibaizhu Powder against HRV Infection of Intestinal Mucosal Epithelial Cells of Suckling Mice

目的探讨七味白术散对人类轮状病毒(HRV)感染乳鼠小肠黏膜上皮细胞的保护作用。方法 5日龄NIH乳鼠经口感染HRV建立乳鼠腹泻模型,随机分为4组:正常组、模型组、七味白术散组、病毒唑组,各组乳鼠分别经口滴入相对应的治疗药物,3次/d(正常化与模型组给生理盐水)。分别于治疗后5d处死乳鼠,取乳鼠小肠粪便检测HRV,HE染色观察小肠黏膜病理变化。结果七味白术散治疗5d乳鼠肠粪便中HRV清除率明显优于模型组(P<0.05);七味白术散组乳鼠小肠黏膜上皮细胞病变明显减轻,与模型组、病毒唑组比较差异均有统计学意义(P<0.05)。结论七味白术散能清除感染乳鼠肠道中的HRV,明显改善HRV感染乳鼠小肠黏膜上皮细胞病变。

Objective To investigate the protective effect of Qiweibaizhu Powder against HRV infection of intestinal mucosal epithelial cells of suckling mice. Methods The 5-day-old NIH mice were made HRV diarrhea model by oral infection, and randomly divided into six groups： normal group （NG）, model group （MG）, Qiweibaizhu Powder group （BG）, ribavirin group （ZG）, suckling mice in each group were instilled corresponding drugs 3 times/d （NG and MG with normal saline） through the mouth. 5 d after treatment, suckling mice were killed, small intestine stool was taken to test HRV, and pathological changes in small intestinal mucosa were observed by HE staining. Results Intestinal faeces HRV clearance of BG was significantly better than MG （P 〈0.05）. Pathological changes of intestinal epithelial cells of BG were significantly reduced, and the differences were statistically significant compared with MG and ZG （P 〈 0.05）. Conclusion Qiweibaizhu Powder can remove HRV in the intestinal tract of suckling mice, improve the lesions of intestinal mucosal epithelial cells of suckling mice infected by HRV.