二仙汤及其组方中药对幼年大鼠肾上腺雌激素受体表达的影响

Effects of Er-xian Decoction and Its Component Herbs on Estrogen Receptors in Adrenal Development Rats

目的通过观察二仙汤及其组方中药对幼年大鼠肾上腺雌激素受体(ER)表达的影响,探讨其对肾上腺功能的影响机制。方法幼年SD大鼠54只,分为正常对照、阳性对照、二仙汤、淫羊藿、仙茅、巴戟天、当归、黄柏和知母9组,各组给药6d后麻醉大鼠,取血清测量睾酮的含量,分离摘取双侧肾上腺,称重后常规石蜡包埋切片,行免疫组织化学染色。结果阳性对照组、二仙汤组肾上腺系数增加,二仙汤组、淫羊藿组、仙茅组和知母组血清睾酮水平降低,以二仙汤组最明显(P<0.01)。ERα主要分布于肾上腺皮质球状带和束状带细胞的细胞质中,二仙汤组平均光密度值升高,而阳性对照组下降;ERβ主要分布于肾上腺皮质球状带和束状带细胞的细胞核中,二仙汤组、淫羊藿组、仙茅组和知母组平均光密度值与阳性对照组比较下降。结论二仙汤及其方中温肾药(淫羊藿、仙茅)和滋阴药(知母)通过影响不同类型ER在肾上腺皮质中的分布发挥植物雌激素样作用。

Objective To explore the possible functional mechanism of Er-xian Decoction and its component herbs on the expression of extrogen receptor （ER） in adrenal development rats. Methods Fifty-four development female SD rats were randomly divided into nine groups： normal control group （A）, positive control group （B）, Er-xian Decoction group （C）, Herba Epimedii group （D）, Rhizoma Curcuh＇ginis group （E）, Radix Morindae Officinalis group （F）, Radix Angelicae Sinensis group （G）, Cortex PheUodendri group （H）, Rhizoma Anemarrhenae group （I）. After the rats were administrated for six days, serum were got to measure testosterone （T）. Bilateral adrenal gland was weighed and embedded by paraffin. Mean optical density （MOD） were studied by the immunohistochemical method and image analyzing system. Results The adrenal coefficient was increased in group B and C, and the level ofT was decreased in group C, D, E and I. ERα was mainly observed in the cytoplasm of zona glomerulosa and zona fasciculate. MOD in group C was increased while declined in group B. ERβ was mainly observed in nucleus of zona glomenalosa and zona fasciculate. MOD in group C, D, E and I was Jess than group B. Conclusion Er-xian Decoction and its component herbs of warming Shen （Herba Epimedii, Rhizoma Curculiginis） and nourishing Yin （Rhizoma Anemarrhenae） play a phytoestrogen role by influencing the expression of ERo and ER β in the adrenal cortex.