重组人Jagged1蛋白促进细胞因子对脐血干细胞的扩增

Recombinant human Jagged 1 protein enhances cytokine induced expansion of umbilical cord blood stem cells

目的探讨重组人Jagged1蛋白对脐血干细胞体外扩增的促进作用。方法利用免疫磁珠法分离人脐带血CD34+细胞,并将其分为4组,分别为:对照组、Jagged1蛋白组、细胞因子组、Jagged1蛋白+细胞因子组。培养6d后,通过台盼蓝染色和MTT等方法检测各组细胞的生长状况;同时采用RT-PCR方法检测干细胞表面标志物CD34+的表达情况。结果以上4组细胞在体外增殖6d后的细胞浓度分别为(0.4±0.1)、(1.3±0.5)、(5.0±0.8)和(10.2±1.5)×104.mL-1;RT-PCR检测显示,各组细胞扩增6d后均有CD34+表达。结论Jagged1蛋白能够促进脐血干细胞的体外扩增,而且Jagged1蛋白与其他促进细胞增殖的因子配伍对脐血干细胞在体外的扩增作用更加明显。

Objective To investigate the effects of recombinant human Jagged 1 on the cytokine induced expan- sion of umbilical cord blood stem cells. Methods CD34 ＋ cells from umbilical cord blood were isolated by MACS sys- tem, and divided into 4 groups cultured in following conditions ： （1）Control group, （2） Jagged 1 protein, （3） Cell factor, （4） Jagged 1 protein and cell factor. Cell proliferation was examined by Trypan blue staining and MTF method after 6 days. Meanwhile, the expression of stem cell surface marker CD34 gene was examined by RT - PCR. Results Cell concentra- tions were 0. 4 ±0. 1, 1.3 ±0. 5, 5.0 ±0. 8 and 10. 2 ± 1.5（×10^4·mL-1） in the four groups, respectively. Furthermore, expression of CD34 gene was detected in all the 4 groups. Conclusion Jagged 1 enhances the expansion of umbilical cord blood stem cells in vitro. It is more effective when Jagged 1 works together with other classic growth factors.