醋酸杆菌产乙醇脱氢酶发酵条件的研究

Study on the Fermentation Conditions of Acetobacter sp. to Produce Alcohol Dehydrogenase

选择一株醋酸杆菌Acetobacter sp.CCTCC M209061,通过单因素和正交实验确定醋酸杆菌产乙醇脱氢酶(Alcohol Dehydrogenase,ADH)的最优发酵条件。结果表明,产ADH的最优培养条件为温度30℃、pH值6.0、通气量为100 mL/250 mL、接种量为8%,在此条件下发酵培养24 h,发酵液酶活可达0.340 U/mL。

Acetobacter sp. CCTCC M209061 was selected in the experiment. Its optimum fermentation conditions to produce ADH were determined by single factor experiments and orthogonal experiments as follows： temperature was at 30℃, pH value was 6.0 and air flow wasl00 mL/250 mL, and inoculating quantity was 8 %. Under the above conditions, the enzyme activity of fermenting broth could reach up to 0.340 u/mL after 24 h fermentation.