辽东楤木根皮的质量标准研究

STUDY ON THE QUALITY STANDARD OF ROOT BARK OF ARALIA ELATA

目的:建立辽东楤木根皮的质量标准。方法:采用薄层色谱法对辽东楤木根皮进行定性鉴别;高效液相色谱(HPLC)法对其所含主要有效成分楤木皂苷A进行含量测定。结果:TLC法可检出辽东楤木根皮中楤木皂苷A的特征斑点;楤木皂苷A在6.325～25.30μg范围内与峰面积具有良好的线性关系(r=0.9999),平均回收率96.63%,RSD为1.23%。结论:该方法灵敏、准确、专属性强、重现性好,可用作辽东楤木根皮的质量控制。

Objective：To establish the quality standard of root bark of Aralia elata.Methods：Aralia elata was identified by TLC;Araloside A in root bark of Aralia elata was detarmined by HPLC.Results：Araloside A could be identified by TLC;the calibration curve showed good linearity over the range of 6.325 ～25.30μg （r= 0.9999） and average recovery was 96.63% with RSD of 1.23%.Conclusion：This method is sensitive,accurate,specific and reproducible,it can be used for quality control of Aralia elata.