早期静脉注射特布他林对大鼠急性肺损伤的影响

The Effect of Injection of Terbutaline Early on Rats with Acute Lung Injury

目的观察早期静脉注射特布他林注射液对大鼠急性肺损伤(ALI)的影响。方法将40只SD大鼠随机分为4组:生理盐水对照组(A组)、ALI组(B组)、ALI+特布他林治疗组(C组)、ALI+特布他林+哇巴因干预组(D组),每组10只。各组大鼠分别以10%水合氯醛3.5μL.g-1腹腔麻醉。麻醉成功后,A组大鼠采用尾静脉注射生理盐水5mg.kg-1,1h内注完;B组大鼠采用尾静脉注射脂多糖(LPS)5mg.kg-1(融于1mL生理盐水中),分4次给药,1h内注完;C、D2组大鼠采用尾静脉注射LPS5mg.kg-1(融于1mL生理盐水中),分4次给药,1h内注射完后,再注射硫酸特布他林注射液5.0μL.g-1体质量,1h内注射完后。然后将大鼠头部抬高45°,光源照射颈部直视下行气管插管,插管成功后,A、B、C3组气管内滴注林格氏液1mL.kg-1,D组气管内滴注含哇巴因10-3mol.L-1的林格氏液1mL.kg-1。通过建立LPS诱导的大鼠急性肺损伤模型,对各组大鼠肺组织病理学评分,观察各组大鼠的肺湿干重比(W/D)、肺组织Na+-K+-ATP酶的活性,并测定各组大鼠肺泡灌洗液中的细胞数、中性粒细胞计数及蛋白含量。结果A组肺组织病理学评分值、W/D值、细胞计数、中性粒细胞百分比、蛋白含量均明显低于B、C、D3组(均P<0.05),B、D2组上述指标均明显高于C组(均P<0.05),B组上述指标与D组比较差异无统计学意义(P>0.05)。A组肺组织Na+-K+-ATP酶的活性明显高于B、C、D3组(P<0.05),B、D2组均明显低于C组(均P<0.05),B组与D组比较差异无统计学意义(P>0.05)。结论早期静脉注射特布他林可通过上调肺组织Na+-K+-ATP酶的活性来促进肺泡上皮细胞对液体重吸收,从而控制大鼠急性肺损伤的进展。

Objective To observe the effect of intravenous injection of terbutaline early on the rats with acute lung injury. Methods Forty SD rats were randomly assigned into 4 groups： NS control group（group A）, ALI group（group B）, ALI＋ terbutaline treatment group （group C） and ALI＋terbutaline＋ouabain intervention group （group D） with 10 in each group. The rats of all groups were respectively anesthetized by intraperitoneal injection of 10% chloral hydrate （3.5uL. g-1）. After being anaesthetized successfully, the rats in group A were injected with 5 mg . kg-1 NS through the tails vein within 1 hour; the rats in group B were injected with 5 mg . kg-1 LPS （which dissolved in 1 mL of NS） throgh the tails vein in 4 times within 1 hour; the rats in group C and D were injected with 5 mg . kg-1 LPS （which dissolved in 1 mL of NS） through the tails vein in 4 times within 1 hour. Then the rats were injected with 5.0 uL . g-1 terbutaline within 1 hour. Then elevated the rats head to 45 degrees,the tracheal intubation was performed under lights illuminating the rats neck,after intubated successfully, 1 mL . kg-1 Ringer＇s solution was dripped into trachea in groups A,B and C,and D with 1 mL . kg-1 Ringer＇s solution which contain 10-3 mol . L- 1 ouabain. To establish Lipopolysaccharide-induced model of acute lung injury in rats,the pathomorphologic scores of lung injury, the wet to dry weight ratio （W/ D）,and the activity of Na＋-K＋-ATPase were determined after 6 hours, and bronehoalveolar layage fluid （BALF） cells count,the percentage neutrophilicgranulocyte （PMN）,BALF concentration of protein were determined too. Results The pathomorphologic scores of lung injury,the wet to W/D,BALF cells count, the PMN,BALF concentration of protein in group A were significantly lower than that in group B,C,and D（all P〈0.05） ,the above mentioned parameters in group B and D were higher than those in group C（all P〈0.05）,but there were no significant differences between groups B and D（P〈0.05）. the activity of Na＋-K＋-ATP ase in group A were significantly lower than that in groups B,C,D（P〈0.05） ,the activity of Na＋-K＋-ATP ase in groups B and D were higher than those in group C（all P〈0.05）, but there were no significant differences between group B and D （P〉0.05）. Conclusion The intravenous injection of terbutaline early can promote alveolar epithelial fluid reabsorption in rats with ALI via the upregulation of the activity of Na＋-K＋-ATP ase in lung,and the effect could control the further development of ALI.