重组人BMP-2对人肝癌SSMC-7721细胞迁移和侵袭能力的影响

The Effect of Recombinant Human Bone Morphogenetic Protein-2 on Migration and Invasive Ability of Human Hepatoma SMMC-7721 Cells

目的观察重组人骨形成蛋白2(rhBMP-2)对人肝癌SMMC-7721细胞迁移和侵袭能力的影响。方法以人肝癌SMMC-7721细胞株为研究对象,取对数期生长的SMMC-7721细胞随机分为4组:rhBMP-2200、400、600μg.L-1组和对照组。rhBMP-2200、400、600μg.L-1组分别加入rhBMP-2200、400、600μg.L-1,对照组加入2mLRPMI1640培养基。分别于12、24、48h后,用细胞划痕法检测细胞体外迁移能力,Transwell小室测定法检测细胞体外侵袭能力。结果rhBMP-2200、400、600μg.L-1组12、24、48hSMMC-7721细胞迁移率与对照组比较差异均有统计学意义(均P<0.05),且rhBMP-2200、400、600μg.L-1组各组间差异具有统计学意义(P<0.05)。rhBMP-2200、400、600μg.L-1组12、24、48hSMMC-7721细胞穿膜数与对照组比较差异均有统计学意义(均P<0.05),rhBMP-2200、400、600μg.L-1组各组间差异具有统计学意义(P<0.05)。rhBMP-2200、400、600μg.L-1组12、24hSMMC-7721细胞迁移率、细胞穿膜数与48h比较差异均有统计学意义(均P<0.05),浓度与时间无交互作用。结论BMP-2可增加人肝癌SMMC-7721细胞的侵袭和迁移能力,且呈时间-浓度依赖性;阻滞BMP-2的表达可能成为抑制肝癌转移的一个潜在靶点。

Objective To observe the effect of recombinant human bone morphogenetic protein 2 （rhBMP-2） on migration and invasive ability of human hepatoma SMMC-7721 cells. Methods Human hepatoma SMMC-7721 cells in logarithmic phase growth cells were randomly divided into the following 4 groups, control group, rhBMP-2 200,400,600 ug . L-1 group. The control group was Join 2 mL RPMI 1640 medium,200,400,600ug . L-1 rhBMP were accordingly added respectively. At the varied experimental phases of 12,24,48 hours,the migration ability of the cells was respectively detected by scarification assay,and invasive ability by Transwell chamber invasion assay. Results In respect of both the migration rate and trans-membrane-cells of the SMMC-7721 cells which were detected respectively at 12, 24, 48 hours of the experimentation, significant difference was found between the control group and the rh BMP-2 200,400,600 ug . L-1 group （all P〈0.05） ;and marked difference was also found between the rhBMP-2 200,400,600ug . L-1 groups（P〈0.05）. For the rhBMP-2 200,400,600 ug . L-1 groups, both the migration rate and trans-membrane-cells of the SMMC-7721 cells detected at 12,24 hours were remarkably different from those detected at 48 hours（all P〈0.05）. Conclusion BMP-2 could increase the invasion and migration ability of human hepatoma SMMC-7721 cells in a dose-time-dependent pattern. Blocking the expression of BMP-2 protein may be a potential target for inhibiting hepatoma metastasis.