肝组织CTGF、TGF-β_1的表达与肝纤维化关系的实验研究

Relationships between Levels of CTGF、TGF-β_1 Gen Expressions in Liver Tissues and Fibrosis Pathology

目的:探讨免疫性肝纤维化肝组织结缔组织生长因子(CTGF)与转化生长因子β1(TGF-β1)的表达与肝纤维化的关系。方法:用猪血清大鼠腹腔内注射复制免疫损伤性肝纤维化模型,免疫攻击16周后处死动物,行肝组织病理分析,免疫组化法检测肝内CTGF、TGF-β1表达。结果:16周后模型组大鼠形成典型的肝纤维化,肝内CTGF与TGF-β1表达均显著增强,同时二者阳性分布部位相近,表达程度呈正相关性;且CTGF表达与组织病理上肝纤维化变化呈正相关性。结论:实验性肝纤维化过程中CTGF与TGF-β1促进肝纤维化的进展。

Objective：To investigate the relationship between fibrosis pathology and the levels of CTGF,TGF-β1 gen expressions in liver tissues. Methods： All rats were given subcutaneous injection of pig serum after 16 weeks, the degrees of fibrosis was detected by histopathology under optical microscopy. The expressions of CTGF, TGF-β1 were measured by immunochemical techniques. Results：The model group had shown typical hepatic fibrosis. The expressions of TGF β1 , connective tissue growth factor increased obviously. Conclusion：The study indicates that TGF-β1 and CTGF accelerate the development of experiment liver fibrosis.