LPS刺激对人结肠癌SW480、肺癌A549细胞HMGB1以及HBD-1表达的影响

Effect of LPS stimulation on expression of HMGB1 and HBD-1 in SW480 cells and A549 cells

目的观察脂多糖(LPS)刺激对SW480、A549细胞HMGB1、HBD-1表达的影响。方法以浓度为5、10、50和100 ng/μl LPS刺激SW480、A549细胞24 h后,提取细胞RNA,并用RT-PCR法检测HMGB1以及HBD-1基因mRNA的表达水平。结果SW480以及A549细胞,HMGB1以及HBD-1均有较高的表达。在SW480细胞中,不同的LPS刺激浓度下,5ng/μl LPS即可上调HMGB1基因mRNA的表达,但其表达量并不随LPS浓度的增高而有所增加;而同样刺激条件下HBD-1基因mRNA表达水平未见明显变化(P>0.05)。在A549细胞中,不同的LPS刺激浓度下HMGB1以及HBD-1基因mRNA表达量未见明显变化(P>0.05)。结论HMGB1及HBD-1固有表达于肠道及呼吸道上皮细胞。LPS能上调SW480细胞HMGB1的表达,其表达量无浓度依赖性,但LPS不能上调HBD-1的表达。在A549细胞,LPS不能诱导HMGB1以及HBD-1的表达。

Objective To investigate the effects of LPS on HMGB1 and HBD-1 expression in SW480 and A549cells. Methods Upon the concentration of 5, 10, 15, 100 μg/mL LPS exposure in 24h, the RNA of SW480 and A549 cells were extracted and the HMGB1 and HBD-1 expression level were identified by reverse transcriptase-polymerase chain reaction （RT-PCR）. Results Both in SW480 and A549 cells, HMGB1 and HBD-1 had a higher expression. Under the different concentrations of LPS stimulation, in SW480 cells, 5 ng/μL LPS could increase the expression of HMGB1 gene mRNA, but its expression level was not increased following the increasing concentration of LPS. Different concentrations seemed not to increase the HBD-1 gene expression levels （P〉0.05）. In A549 ceils, various LPS concentrations did not change the expression level of HBD-1 and HMGB1. Conclusion HMGB1 and HBD-1 inherently express in epithelial cells of the gastro-intestinal and respiratory tract. LPS could increase the expression of HMGB1 gene mRNA, but its expression level is not increased following the increasing concentration of LPS, while different concentrations of LPS stimulation have no significant effect on the expression of HBD-1 in the SW480 cells. In A549 cells, different concentrations of LPS stimulation have no significant effects on the expression of HBD-1 and HMGB1.