摘要
目的了解临床痰液及尿液标本中分离的产超广谱β-内酰胺酶(ESBLs)大肠埃希菌的耐药特点及差异,以便合理使用抗菌药物。方法收集临床2008年9月至2009年8月痰(含咽拭子)及中段尿标本进行细菌鉴定及药物敏感性测定。根据是否产ESBLs将检测出的大肠埃希菌分为ESBLs阳性组和ESBLs阴性组,分别统计比较两种标本中ESBLs阳性组和ESBLs阴性组大肠埃希菌对抗菌药物的耐药特点及差异。结果中段尿检出率为50.2%(101/201),痰液检出率为62.6%(117/187)。中段尿分离的ESBLs阳性大肠埃希菌对大部分药物耐药率均高于痰液中ESBLs阳性的大肠埃希菌;而中段尿分离的ESBLs阴性大肠埃希菌对大部分药物耐药率均低于痰液中ESBLs阴性的大肠埃希菌。结论痰液标本中ESBLs阳性大肠埃希菌检出率高于尿液标本,并且相对于痰液中的大肠埃希菌,尿液标本中ESBLs阳性大肠埃希菌对大部分药物呈高耐药趋势,而尿液标本中ESBLs阴性大肠埃希菌对大部分药物呈低耐药趋势。
Objective To investigate the antimicrobial resistance of escherichia coli（E, coli）, which produces extended spectrum β-1actamases（ESBLs）,so as to guide the rational use of antimierobial agents. Methods Strains of E. colt isolated from urine and sputum samples from Sept. 2008 to Aug. 2009 were collected. E. colt was divided into ESBLs positive group and ESBLs negative group according to the fact whether producing or not producing ESBLs. Bacterial identification and antlmicrohial susceptibility tests were performed by Hunan TDR semi-automatic system, and drug resistant rate of E. colt isolated from urine and sputum samples were compared statistically. Results The rate of ESBLs positive E. colt was 56.2 %（218/388） in the samples isolated from urine and sputum with the rate being 50.2 %（101/201） in urine and 62.6%（117/187） in sputum. To many antimicrobial gents,ESBLs positive E. colt isolated from urine samples have higher resistant rates than that from sputum samples, while ESBLs negtive E. colt isolated from urine have lower resistant rates than that from sputum samples. Conclusion The rate of ESBLs positive E. colt in sputum was higher than that in urine. Furthermore,ESBLs positive E. colt isolated from urine sam- ples show a higher resistant tendency than the one isolated from sputum samples to many antimicrobial agents,while ESBLs negative E. colt isolated from urine is of lower resistant tendency than the one isolated from sputum samples to many antimicrobial agents.
出处
《检验医学与临床》
CAS
2010年第9期811-813,共3页
Laboratory Medicine and Clinic
